GNAT3 (Center) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IHC, WB
Recommend Dilution	Peptide ELISA: 1/1000. Western Blot: 1/1000. Flow Cytometry: 1/10-1/50. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic peptide between 78-105 amino acids from the Central region of Human GNAT3
Specificity	This antibody recognizes Human GNAT3 (Center).
Formulation	PBS containing 0.09% (W/V) Sodium Azide as preservative State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein A column, followed by peptide affinity purification
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	G protein subunit alpha transducin 3
Database Link	Entrez Gene 346562 Human UniProt ID A8MTJ3
Background	Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.
Synonyms	Gustducin alpha-3 chain, G protein G(t) subunit alpha-3
Note	Molecular Weight: 40357 Da
Reference Data
Protein Pathways	Taste transduction

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