ACK1 (TNK2) pTyr284 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1:500~1:1000. Immunohistochemistry on paraffin sections: 1:50~1:100. Immunofluorescence: 1:100~1:200.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y(p)-V-M) derived from Human ACK1 (KLH-conjugated)
Specificity	The antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Affinity chromatography using epitope-specific peptide
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	120 kDa
Gene Name	tyrosine kinase non receptor 2
Database Link	Entrez Gene 10188 Human UniProt ID Q07912
Background	Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes.
Synonyms	ACK; ACK-1; ACK1; FLJ44758; FLJ45547; p21cdc42Hs
Reference Data

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