PGP9.5 (UCHL1) Mouse Monoclonal Antibody [Clone ID: 13C4]

Specifications

Product Data
Clone Name	13C4
Applications	IF, IHC, WB
Recommend Dilution	ELISA: Use BSA free Antibody for Coating. Western blot: 0.5-1 µg/ml. Immunofluorescence: 1-2 µg/ml. Immunohistochemistry on Frozen Sections. Positive Control: Cerebellum.
Reactivity	Bovine, Canine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Native Human UCHL1 (PGP9.5) protein from brain
Specificity	Recognizes Protein Gene Product 9.5 (PGP9.5). This Monoclonal Antibody reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). BM160 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES. Also stains neuroendocrine cells in human adult gut (unlike 31A3). Clones 31A3 and 13C4 each recognize a different epitope towards the N-terminus of the protein. Cellular Localization: Cytoplasmic. Endoplasmic Reticulum membrane.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Bioreactor concentrate by Protein A/G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	20-30 kDa
Gene Name	ubiquitin C-terminal hydrolase L1
Database Link	Entrez Gene 7345 Human UniProt ID P09936
Background	PGP9.5 is a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway. Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Synonyms	UCH-L1, PGP 9.5, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5
Reference Data

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