IGF1 Receptor (IGF1R) (alpha chain) Mouse Monoclonal Antibody [Clone ID: 1H7]

Specifications

Product Data
Clone Name	1H7
Applications	FC, IHC, WB
Recommend Dilution	Western Blot. Immunohistochemistry on Paraffin Sections: 1/10-1/40. Requires Protein digestion pre-treatment of paraffin sections (e.g. trypsin or pronase). Flow Cytometry: Use 10 µl of 1/10 diluted antibody to label 106 cells in 100 µl. Recommended Positive Control: Pancreas, placenta.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human placental IGF-I receptor.
Specificity	This antibody recognizes CD221, a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane betasubunits. Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221. We recommend the use of BM2226LE for this purpose.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from tissue culture supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	insulin like growth factor 1 receptor
Database Link	Entrez Gene 3480 Human UniProt ID P08069
Background	There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor (also known as CD221) prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
Synonyms	IGF-I receptor, IGF1 Receptor
Reference Data

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