EPCAM Mouse Monoclonal Antibody [Clone ID: HEA125]
CAT#: BM2274
EPCAM mouse monoclonal antibody, clone HEA125, Purified
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CNY 4,350.00
货期*
5周
规格
Cited in 1 publication. |
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Specifications
Product Data | |
Clone Name | HEA125 |
Applications | IF, IHC, WB |
Recommend Dilution | Immunofluorescence Microscopy. Cell Sorting. Cytological material. Immunoblotting. Immunohistochemistry on Frozen Sections and Paraffin-Embedded Sections. With paraffin embedded sections, protease pre-treatment is required prior to antibody application. Recommended Dilutions: Dilute at least 1/10 with PBS, pH 7.4, for Imunohistochemical application. Incubation time: 1 h at RT; extended with paraffin. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | HT-29 colon carcinoma cell line |
Specificity | The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116). Polypeptide Reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM). |
Formulation | PBS, pH 7.4 State: Purified State: Lyophilized purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide Label: Restore in 1.0 ml distilled water |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage Condition | Prior to reconstitution store at 2-8°C. |
Gene Name | epithelial cell adhesion molecule |
Database Link | |
Background | Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium. |
Synonyms | Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18 |
Reference Data |
Citations (1)
The use of this Antibodies has been cited in the following citations: |
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Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway.
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International journal of oncology
,PubMed ID 22020958
[EPCAM]
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