CD62E (SELE) (+CD62P) Mouse Monoclonal Antibody [Clone ID: 1.2B6]

Specifications

Product Data
Clone Name	1.2B6
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10µl of neat antibody to label 1 x 10e6 cells in 100µl.
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Human E-Selectin (ELAM-1). Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Specificity	This antibody recognizes the CD62E and CD62P cell surface antigens. Although previously thought to recognise only Human CD62E, recent data (Goda et al. 2003) shows that this antibody also recognizes Human CD62P, binding to a common epitope shared by these members of the selectin family.
Formulation	PBS, pH 7.4 Label: FITC State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	selectin E
Database Link	Entrez Gene 6401 Human UniProt ID P16581
Background	CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x.
Synonyms	SELE, ELAM1, LECAM2
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs)

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