Collagen II (COL2A1) Mouse Monoclonal Antibody [Clone ID: 2G2/49 (COLL-II)]

CAT#: BM332

Collagen II (COL2A1) mouse monoclonal antibody, clone 2G2/49 (COLL-II), Ascites



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CNY 9,600.00


货期*
4周

规格
    • 100 ul

Cited in 8 publications.

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Specifications

Product Data
Clone Name 2G2/49 (COLL-II)
Applications ELISA, IF, WB
Recommend Dilution ELISA: 1/100-1/500.
Western blotting: 1/1000-1/2000.
Immunofluorescence: Neat-1/5. Suitable for use on Fresh, Frozen or Acetone fixed material. Mild pepsin digestion is recommended to enhance staining.
Reactivity Bovine, Canine, Human, Mouse, Porcine, Rat, Sheep
Host Mouse
Clonality Monoclonal
Immunogen Human cartilage specific CNBr-cleaved Collagen II
Specificity Reacts with both pepsin solubilised and CNBr-cleaved Human and Bovine Collagen type II.
No-cross reactivity is seen with types I, III, V or IX.
Species Cross-Reactivity: Rat, Bovine, Sheep, Mouse, Dog, Pig.
Formulation State: Ascites
State: Liquid Ascites without preservatives
Stabilizer: None
Preservative: None
Conjugation Unconjugated
Storage Condition Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Gene Name collagen type II alpha 1 chain
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues.
Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Synonyms COL2A1, Alpha-1 type II collagen
Reference Data
Protein Pathways ECM-receptor interaction, Focal adhesion
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (8)

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