Corticotropin Releasing Factor (CRH) Mouse Monoclonal Antibody [Clone ID: 5D5]

Specifications

Product Data
Clone Name	5D5
Applications	ELISA, IHC
Recommend Dilution	ELISA: High binding constant allows this antibody to be used in a Sandwich ELISA with clone number 4H9 (BM357); Sensitivity: 25 pg/ml. Immunohistochemistry on Paraffin Sections: 1-10 µg/ml. Protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase is required. Antigen retrieval using heat treatment (Sodium citrate buffer pH 6.0) prior to staining of paraffin sections is required.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic Human Corticotropin Releasing Factor
Specificity	Recognises a different epitope than clone 4H9 (BM357). 5D5 mAb binding decreases only partly after CA treatment. It is possible that the antigen determinant is exposed more openly after DEP modification of histidine residues, whereas this determinant becomes barely accesible after lysine modification. Human serum, containing CRF-binding protein, inhibits the binding of CRF to this antibody.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	corticotropin releasing hormone
Database Link	Entrez Gene 1392 Human UniProt ID P06850
Background	CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).
Synonyms	CRF, CRH, Corticotropin-Releasing Hormone, Corticotropin-Releasing Factor
Reference Data

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