Desmoplakin (DSP) (DP1+DP2) Mouse Monoclonal Antibody [Clone ID: DP2.15]

Specifications

Product Data
Clone Name	DP2.15
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections: Dilute 1/10 with PBS, pH 7.4 Incubation Time: 1 h at RT.
Reactivity	Amphibian, Bovine, Canine, Chicken, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Bovine Desmoplakin 1 and 2
Specificity	This antibody reacts to Desmoplakin 1&2. It shows distinct punctate membrane staining of different epithelia. Tumors Specifically Detected: Primary and metastatic carcinoma and meningioma. Polypeptides Reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000). Reactivity on Tested Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431; Rat MHC1C1 cell line; Bovine cells: MDBK, BMGE.
Formulation	Final solution contains PBS, pH 7.4 State: Purified State: Lyophilized purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore in 1 ml distilled water
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	desmoplakin
Database Link	Entrez Gene 306871 RatEntrez Gene 1832 Human UniProt ID P15924
Background	Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
Synonyms	DP, DSP, 250/210 kDa paraneoplastic pemphigus antigen
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Arrhythmogenic right ventricular cardiomyopathy (ARVC)

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