Cd68 Mouse Monoclonal Antibody [Clone ID: ED1]
CAT#: BM4000S
Cd68 mouse monoclonal antibody, clone ED1, Purified
Size: 250 ug
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CN¥ 6,215.00
货期*
4周
规格
Cited in 5 publications. |
Specifications
Product Data | |
Clone Name | ED1 |
Applications | FC, IF, IHC, IP, R, WB |
Recommend Dilution | RIA. Western blotting. Immunoprecipitation. Immunofluorescence. Flow Cytometry: Use 10 µl of 1/25-1/100 diluted CD68 antibody to label 106 cells in 100 µl (Membrane permeabilisation is required for best results). Immunohistology on Frozen Sections. Immunohistology on Paraffin Sections: 1/50-1/100. Requires protein digestion pretreatment of Paraffin sections with Pronase or Trypsin. |
Reactivity | Bovine, Rat |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Rat spleen cells. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line. |
Specificity | CD68 Antibody (Cat#BM4000) recognises a single chain glycoprotein of 110 kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. |
Formulation | PBS, pH 7.4 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | Cd68 molecule |
Database Link | |
Background | The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. |
Synonyms | Gp110, Macrosialin, Macrophage marker |
Note | Studies have shown that the antigen recognised by ED1 is the Rat homologue of Human CD68. |
Reference Data |
Citations (5)
The use of this Antibodies has been cited in the following citations: |
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Cirrhotic Cardiomyopathy Following Bile Duct Ligation in Rats—A Matter of Time?
,null,
International Journal of Molecular Sciences
,PubMed ID 37175858
[Cd68]
|
Intermittent Hypoxia Contributes to the Lung Damage by Increased Oxidative Stress, Inflammation, and Disbalance in Protease/Antiprotease System
,Tuleta I, Stvckigt F, Juergens UR, Pizarro C, Schrickel JW, Kristiansen G, Nickenig G, Skowasch D,
Lung
,PubMed ID 27738828
[CD68]
|
In Vivo Imaging of Fluorescent Probes Linked to Antibodies Against Human and Rat Vascular Endothelial Growth Factor
,Meyer JH, Cunea A, Licha K, Welker P, Sonntag-Bensch D, Wafula P, Dernedde J, Fimmers R, Holz FG, Schmitz-Valckenberg S,
Invest Ophthalmol Vis Sci.
,PubMed ID 26927569
[CD68]
|
In vivo imaging of a new indocyanine green micelle formulation in an animal model of laser-induced choroidal neovascularization
,Meyer J, Cunea A, Sonntag-Bensch D, Welker P, Licha K, Holz FG, Schmitz-Valckenberg S,
Invest. Ophthalmol. Vis. Sci.
,PubMed ID 25190666
[CD68]
|
Low-dose indomethacin after ischemic acute kidney injury prevents downregulation of Oat1/3 and improves renal outcome
,Schneider R, Meusel M, Renker S, Bauer C, Holzinger H, Roeder M, Wanner C, Gekle M, Sauvant C,
Am. J. Physiol. Renal Physiol.
,PubMed ID 19794109
[CD68]
|
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