Adgre1 Rat Monoclonal Antibody [Clone ID: CI:A3-1]

Specifications

Product Data
Clone Name	CI:A3-1
Applications	FC, IF
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl. Immunoflourescence.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.
Specificity	This antibody recognises the F4/80 antigen. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (5). We recommende the use of Cat.-No BM4008LE for this purpose.
Formulation	PBS, pH 7.4 Label: PE State: Lyophilized purified Ig fraction Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1 ml distilled water.
Purification	Affinity Chromatography on Protein A
Conjugation	PE
Storage Condition	Prior to reconstitution and following reconstitution store at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	adhesion G protein-coupled receptor E1
Database Link	Entrez Gene 13733 Mouse UniProt ID Q61549
Background	Murine F4/80 antigen is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with Human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Synonyms	Emr1, Gpf480
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.