Ly6c1 Rat Monoclonal Antibody [Clone ID: ER-MP20]

Specifications

Product Data
Clone Name	ER-MP20
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/1000) Immunohistochemistry on Paraffin Sections: 0.4 µg/ml (1/500). Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Mouse spleen. Has been described to work in FACS.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse macrophage cell lines.
Specificity	This Monoclonal antibody ER-MP20 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is ideally suitable for the detection of monocytes in bone marrow samples by FACS. ER-MP20 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti F4/80 antibody BM4007) is recommended. ER-MP20 also detects a wide range of endothelial cells. Antigen Distribution on Isolated cells: In bone marrow cells the antigen is found on monoblasts and late CFU-M cells as well as on monocytes. It is also found on granulocytes and a subpopulation of lymphocytes in the peripheral blood. Granulocytic cells show a dull, and monocytic cells a bright antigen surface expression. Lymphoid cells express the antigen only very weakly. Thus, in the bone marrow three useful FACS windows can be defined for cell sorting purposes. Antigen Distribution on Tissue Sections: The antigen is found on macrophage precursor subpopulations in the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Endothelial cells of small vessels in various organs also stain positive with ER-MP20. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen.
Formulation	Stock solution containing PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.05% Kathon as a preservative Label: Biotin State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml IgG
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Gene Name	lymphocyte antigen 6 complex, locus C1
Database Link	Entrez Gene 17067 Mouse UniProt ID P0CW02
Background	Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.
Synonyms	Lymphocyte antigen 6C2, Ly-6C2, Ly6C2, Mouse Macrophage Marker
Reference Data

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