MRP8 (S100A8) Mouse Monoclonal Antibody [Clone ID: S13.67]

Specifications

Product Data
Clone Name	S13.67
Applications	ELISA, FC, IHC, WB
Recommend Dilution	Flow cytometry. ELISA. Dot blots. Immunohistochemistry on acetone-fixed frozen sections at 1-2 µg/ml (1:200 - 1:100) and paraffin sections at 4 µg/ml (1:50), pretreatment is not necessary. Suggested positive control: Human tonsil.
Reactivity	Human, Porcine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Cultured human monocytes, the antigen is MRP8.
Specificity	Clone S13.67 identifies MRP8: The antibody is useful in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. This clone also stains cells in rat spleen, indiating significant cross reactivity with the corresponding rat MRP8. Antigen distribution: Isolated cells - The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3 - 4 days. Tissue sections - In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages increasingly synthesise MRP-8 during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8 / MRP14.
Formulation	PBS, pH 7.2 with 0.05% sodium azide State: Purified State: Liquid
Concentration	0.89 mg/ml
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 2-8°C. After reconstitution, aliquot and freeze stock solution at -20°C. Avoid repeated freezing and thawing.
Gene Name	S100 calcium binding protein A8
Database Link	Entrez Gene 6279 Human UniProt ID P05109
Background	MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.
Synonyms	S100-A8, CAGA, MRP-8, CFAG
Reference Data

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