CD18 (ITGB2) (activation epitope) Mouse Monoclonal Antibody [Clone ID: MEM-148]

Specifications

Product Data
Clone Name	MEM-148
Applications	FC, FN, IP, WB
Recommend Dilution	Flow Cytometry. Immunoprecipitation. Western Blot: Non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peripheral blood mononuclear cells.
Specificity	This antibody recognises an epitope on the CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone MEM-148 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognised by MEM-148 is also exposed during dissociation of the CD11/CD18 by low pH.
Formulation	State: Azide Free State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	integrin subunit beta 2
Database Link	Entrez Gene 3689 Human UniProt ID P05107
Background	CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.
Synonyms	Integrin beta-2, MFI7
Reference Data

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