CD163 Mouse Monoclonal Antibody [Clone ID: 5C6-FAT]

Specifications

Product Data
Clone Name	5C6-FAT
Applications	FC, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections: 0.5 μg/ml (1/400). Immunohistochemistry on Paraffin Sections: 1 μg/ml. Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human placenta. Has been described to work in FACS and Western blots.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human CD163. The epitope has not been further characterized.
Specificity	Clone 5C6 FAT recognizes CD163, a membrane glycoprotein on human monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Antigen distribution on isolated cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets. Antigen Distribution Tissue Sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, andcortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with monoclonal antibody BM4022 (clone 25F9) which is not the case in other tissues. Isolated Cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets.
Formulation	Stock Solution containing PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative Label: Biotin State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD163 molecule
Database Link	Entrez Gene 9332 Human UniProt ID Q86VB7
Background	CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.
Synonyms	M130, Hemoglobin scavenger receptor, Macrophage marker
Reference Data

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