CD71 (TFRC) Mouse Monoclonal Antibody [Clone ID: VIP-1]
CAT#: BM4051
CD71 (TFRC) mouse monoclonal antibody, clone VIP-1, Purified
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CNY 8,588.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | VIP-1 |
Applications | IHC |
Recommend Dilution | Immunohistochemistry on Frozen Sections: 2-4 µg/ml (1/50-1/100). Positive Control: Human tonsil. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | KG-1 cells |
Specificity | Monoclonal antibody VIP-1 recognizes CD71 and is useful for the typing of leukemias and the detection of proliferating cells in frozen tissue sections. Antigen Distribution: Positive on activated T and B cells, macrophages and on proliferating cells. Negative on non-activated lymphocytes, monocytes and granulocytes. |
Formulation | Stock solution contains PBS, pH 7.2 with 0.02% Sodium Azide as preservative and 10 mg/ml BSA as stabilizer. State: Purified State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore with 0.5 ml distilled water (= 0.2 mg/ml stock solution). |
Concentration | 0.2 mg/ml |
Purification | Affinity Chromatography. |
Conjugation | Unconjugated |
Storage Condition | Aliquots of stock solution can be kept frozen at -20°C to -70°C. Do not freeze working dilutions. Avoid repeated freezing and thawing. Stability of stock solution: 1 year at -20°C. |
Gene Name | transferrin receptor |
Database Link | |
Background | CD71 is a homodimeric membrane glycoprotein consisiting of two disulfide-bonded 95kD chains. It is a type II membrane protein with a short aminoterminal cytoplasmic domain of 61 amino acid residues which mediates internalization and recycling, a single transmembrane region of 28 amino acid residues, and a large extracellular transferrin-binding domain of 671 residues. Other significant structural features include N-linked and O-linked glycans and the covalent attachment of palmitic acid to cystein residues close to the cytoplasmic face of the membrane. |
Synonyms | TfR1, p90, Transferrin receptor protein 1 |
Note | Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum |
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