CD71 (TFRC) Mouse Monoclonal Antibody [Clone ID: VIP-1]

Specifications

Product Data
Clone Name	VIP-1
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 2-4 µg/ml (1/50-1/100). Positive Control: Human tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	KG-1 cells
Specificity	Monoclonal antibody VIP-1 recognizes CD71 and is useful for the typing of leukemias and the detection of proliferating cells in frozen tissue sections. Antigen Distribution: Positive on activated T and B cells, macrophages and on proliferating cells. Negative on non-activated lymphocytes, monocytes and granulocytes.
Formulation	Stock solution contains PBS, pH 7.2 with 0.02% Sodium Azide as preservative and 10 mg/ml BSA as stabilizer. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water (= 0.2 mg/ml stock solution).
Concentration	0.2 mg/ml
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Aliquots of stock solution can be kept frozen at -20°C to -70°C. Do not freeze working dilutions. Avoid repeated freezing and thawing. Stability of stock solution: 1 year at -20°C.
Gene Name	transferrin receptor
Database Link	Entrez Gene 7037 Human UniProt ID P02786
Background	CD71 is a homodimeric membrane glycoprotein consisiting of two disulfide-bonded 95kD chains. It is a type II membrane protein with a short aminoterminal cytoplasmic domain of 61 amino acid residues which mediates internalization and recycling, a single transmembrane region of 28 amino acid residues, and a large extracellular transferrin-binding domain of 671 residues. Other significant structural features include N-linked and O-linked glycans and the covalent attachment of palmitic acid to cystein residues close to the cytoplasmic face of the membrane.
Synonyms	TfR1, p90, Transferrin receptor protein 1
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.