TNFRSF1A Mouse Monoclonal Antibody [Clone ID: htr9]

Specifications

Product Data
Clone Name	htr9
Applications	FC, FN, IHC, WB
Recommend Dilution	Flow Cytometry: 10 µg/ml. Immunohistochemistry on Frozen Sections: 2-5 µg/ml on Cryostat Sections. Concentrations < 25 µg/ml may give non specific staining of discrete cells. This putative cross reactivity with nuclear (?) antigens cannot be abrogated by pre- or coincubation with TNF. Fixation: Acetone 5 min 4°C or 2% Paraformaldehyde-Lysine Sodium Periodate. Western blots: 2-20 µg/ml on non-reduced SDS PAGE. The antibody recognizes an epitope conformation which is sensitive to reduction. Enrichment of the antigen is recommended since expression is generally low. Functional Assay: htr9 is useful for studying biological effects of TNF-receptor p55 in vitro. The antibody inhibits the binding of radiolabelled TNF to Human cells expressing the p55 TNF receptor. In order to obtain complete inhibition of TNF binding to the cell surface, 10 µg/ml of htr-9 are required. htr9 itself may have an agonistic effect in assays measuring cytotoxicity, fibroblast growth or IL-6 secretion.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Partially purified preparation of TNF binding protein from HL-60 cells.
Specificity	The antibody reacts with CD120a. Antigen Distribution on Tissue Sections: Immunohistochemical staining in normal tissue is confined to the lymphohistiocytic tissue, which includes the thymus and lymphoid organs such as spleen, tonsils, lymph nodes, mucosa, and associated lymphoid tissue (Ref.7). Expression of p55 and p75 receptors can be detected in different areas where an overlapping is found between TNFR p75 and IL-2 receptor expression. The p75 expression can be detected mainly in the T-cell area whereas the p55 expression is restricted to dendritic reticulum cells in the germinal centers. In non-lymphoid organs (kidney, liver, heart, brain, adrenals, uterus, ovary, testes, prostate, stomach, intestines) htr9 recognizes some interstitial reticulum cells in the kidney only. Cells which are known to respond to TNF namely endothelial cells, smooth muscle cells and fibroblasts did not show expression of p55 and or p75 TNF receptor (Ref.7). Investigations on pathological tissues show a TNFR p75 expression on epitheloid cell granulomas and giant cells in sarcoidosis.
Formulation	PBS, pH 7.2 State: Azide Free State: Liquid purified IgG fraction from Culture Supernatant Stabilizer: None Preservative: None
Concentration	1.0 mg/ml (by Absorbance at 280 nm)
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor receptor superfamily member 1A
Database Link	Entrez Gene 7132 Human UniProt ID P19438
Background	Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF Receptor superfamily along with FAS antigen and CD40. TNF Receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 41BB, which are characterized by cysteine rich motifs in the extracellular domain. While TNF Receptor I and TNF Receptor II share 28% sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways. TNF Receptor I contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein), and subsequent interactions with FADD. TNF Receptor I can also activate the transcription factor NFkB via TRAF2 (TNF Receptor associated factor 2). The cytoplasmic domain of TNF Receptor I can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade. TNF Receptor I is expressed by virtually all nucleated mammalian cells, including hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34 + hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF Receptor I.
Synonyms	Tumor necrosis factor receptor 1, TNF-R1, TNF-RI, TNFR-I, p55, p60, Tnfrsf1a
Reference Data

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