Angiotensin Converting Enzyme 1 (ACE) Mouse Monoclonal Antibody [Clone ID: CG2]

Specifications

Product Data
Clone Name	CG2
Applications	IHC
Recommend Dilution	Suitable for Immunohistochemistry on Paraffin sections: 8 µg/ml (1:50); microwave pretreatment for antigen retrieval is recommended. Suggested positive control: Human lung.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified ACE from human lung and kidney.
Specificity	Angiotensin converting Enzyme (ACE), denatured. The epitope recognized is localized on the NH2-terminal side of the molecule. Topographically the degree of expression correlates with the proportion of smooth muscle cells and elastic fibres of the vessel wall. ACE is predominantely expressed by small muscular arteries and by arterioles, whereas small and large veins do not usually express ACE. However, no ACE could be detected in the endothelial cells of kidney. Constitutively ACE is expressed on endothelial cells in different diseases like hypertension, myocardial infarction, sarcoidosis, diabetes.
Formulation	PBS, pH 7.2 containig 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.1% Kathon as a preservative. State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml
Purification	Protein A chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Gene Name	angiotensin I converting enzyme
Database Link	Entrez Gene 1636 Human UniProt ID P12821
Background	ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.
Synonyms	DCP, DCP1, Angiotensin-converting enzyme
Note	Protocol: Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Place slide in a cuvette with 250ml 0.01M citrate buffer, pH 6.0 3. Heat slide in a microwave oven for 2 x 7min. at 700Watt 4. Leave slide in the buffer for 20min for cooling 5. Wash in distilled water 6. Block endogenous peroxidase 7. Wash in PBS 8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 12. Wash in PBS 13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 14. Wash in PBS 15. Counterstain with Mayer's hemalum For further information and details see technical information
Reference Data

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