Surfactant protein D (SFTPD) (Conformational epitope) Mouse Monoclonal Antibody [Clone ID: IIE11 (SPDE)]

Specifications

Product Data
Clone Name	IIE11 (SPDE)
Applications	ELISA, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections: 2 µg/ml (1/250) Does not react on routinely processed paraffin sections. Recommneded Positive Control: Rat lung. Has been described to work in Western blots.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Rat Surfactant protein D from bronchoalveolar lavage.
Specificity	SPDE recognizes a conformational epitope of SP-D. Clone SPDE is a reclone from clone IIE11, with identical reactivity. Antigen Distribution on Tissue Sections: Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.
Formulation	Stock solution contains PBS, pH 7.2 with 0.09% Sodium Azide as preservative and 5 mg/ml BSA as stabilizer. State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore by adding 0.5 ml distilled water (= 0.5 mg/ml Stock Solution).
Concentration	0.5 mg/ml
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store original vial at 2-8°C and stock solution or aliquots thereof at -20°C. Avoid repeated thawing and freezing.
Gene Name	surfactant protein D
Database Link	Entrez Gene 6441 Human UniProt ID P35247
Background	Surfactant Protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43kDa in its reduced state, 620kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.
Synonyms	Lung surfactant protein D, SP-D, PSP-D, PSPD, SFTP4, Collectin-7, COLEC7
Reference Data

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