CD68 Mouse Monoclonal Antibody [Clone ID: Ki-M7]

Specifications

Product Data
Clone Name	Ki-M7
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl to label 106 cells in 100 µl (Membrane permeabilisation is required). Clone KiM7 has been reported as being also suitable for use in Immunoprecipitation and Immunohistochemistry on Frozen Sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Cell suspensions of Human lymph node
Specificity	CD68 antibody (Clone Ki-M7) Cat.-No BM4111F recognizes the 110KD integral membrane glycoprotein CD68 (Macrosialin) predominantly expressed on the intracellular lysosomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes.
Formulation	PBS Label: FITC State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA Preservative: 0.05% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1 Absorption emission: 495 nm / 528 nm
Purification	Protein A Chromatography
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FEEZE! This product is photosensitive and should be protected from light.
Gene Name	CD68 molecule
Database Link	Entrez Gene 968 Human UniProt ID P34810
Background	The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukaemia studies.
Synonyms	Gp110, Macrosialin, Macrophage marker
Reference Data

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