EMAP II (AIMP1) Mouse Monoclonal Antibody [Clone ID: 546-2]

Specifications

Product Data
Clone Name	546-2
Applications	ELISA, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections: 10 μg/ml (1/40) Immunohistochemistry on Paraffin Sections: 10 μg/ml (1/40). Microwave pretreatment for antigen retrieval is recommended. Recommended Positive Control: Rat spleen. Has been described to work in ELISA and Western Blot.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human EMAPII. The epitope has not been further characterized.
Specificity	This antibody reacts with Endothelial-Monocyte-Activating Polypeptide II (EMAP II).
Formulation	PBS, pH 7.2 containing 0.09% Sodium Azide as preservative State: Purified State: Lyophilized IgG fraction.
Reconstitution Method	Reconstitute by adding 0.5ml distilled water.
Concentration	1.0 mg/ml
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated thawing and freezing.
Gene Name	aminoacyl tRNA synthetase complex interacting multifunctional protein 1
Database Link	Entrez Gene 9255 Human UniProt ID Q12904
Background	EMAPII is a proinflammatory cytokine and chemoattractant of macrophages. The 20kDa mature peptide binds to endothelial cells and leukocytes like macrophages. EMAP II activates endothelial cells, by elevation of cytosolic free calcium concentration, release of von Willebrand factor, induction of tissue factor, and expression of adhesion molecules E-selectin and P-selectin. It further activates resting monocytes by elevating cytosolic free calcium concentration, inducing TNFalpha and tissue factor and stimulating chemotaxis. EMAP II has been shown in a tumor model to sensitize the local tumor vasculature to the action of TNFalpha, rendering resistant tumors sensitive to immunotherapy by TNFalpha.
Synonyms	EMAP-2, EMAP-II, AIMP1
Reference Data

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