Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: V9]

Specifications

Product Data
Clone Name	V9
Applications	IHC, WB
Recommend Dilution	Western blotting. Immunohistochemistry on Frozen Sections: ~1/300 in PBS Immunohistochemistry on Formalin-Fixed, Paraffin Embedded Sections: ~1/50-1/100. Trypsin digestion of Paraffin Sections may be required. An excellent result on paraffin embedded tissues could be obtained with a TUF pretreatment. Positive Control: Sarcoma or Melanoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Vimentin isolated from Cow lens.
Specificity	The antibody clone V9 stains the 57 kD Vimentin band in Immunoblots performed on a lysate of normal Human lymphocytes. It does not stain Mouse or Rat Vimentin. The antibody stains all cells containing Vimentin. Cross reactivity: GFAP: not detectable. Keratin : not detectable. Neurofilamen: not detectable. Desmin: not detectable. Cellular Localization: Cytoplasmic.
Formulation	PBS State: Supernatant State: Liquid purified Supernatant Stabilizer: 1% BSA Preservative: 20 mM Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin is the major subunit protein of the intermediate filaments of mesenchumal cells, and has a molecular weight of 57 kD. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Synonyms	VIM
Reference Data

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