Cingulin (CGN) Mouse Monoclonal Antibody [Clone ID: 139.3.4]

Specifications

Product Data
Clone Name	139.3.4
Applications	IHC, WB
Recommend Dilution	Immunoblotting. Immunohistochemistry on Frozen and Paraffin Sections (only after microwave treatment): Ready-to-use. On cultured cell fixation with paraformaldehyde is recommended for optimal staining.
Reactivity	Bovine, Human, Mouse, Xenopus
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide (aa. 4-24)
Specificity	This monoclonal antibody reacts specifically with Cingulin (a 150 kD polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas), of transitional epithelia (e.g. bladder epithelium), and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina). Negative with endothelia of vessels and capillaries (e.g. in heart and brain). In contrast to Symplekin, using Monoclonal antibody 139.4 Cingulin has been localized exclusively to the tight junction complex. Tested Reactivities on Cultured Cell Lines: Carcinoma cell lines (e.g. MCF-7, Caco-2, PLC, A-431), SV80 (fibroblasts), Glioma; MDBK, MDCK, BMGE.
Formulation	State: Supernatant State: Liquid Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	cingulin
Database Link	Entrez Gene 57530 Human UniProt ID Q9P2M7
Background	Cingulin, a polypeptide of 126.5 kD (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5 (very similar to that of symplekin, another junctional protein).
Synonyms	CGN, KIAA1319
Reference Data

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