VIII Mouse Monoclonal Antibody [Clone ID: B62-FE2]

Specifications

Product Data
Clone Name	B62-FE2
Applications	ELISA, EM, WB
Recommend Dilution	Phage Display (immunoassays for the identification of recombinant antigen- or antibody-phages): Detection limit: 107 phage particles. Immunoblotting. Immunoelectron Microscopy. ELISA: 1/500.
Reactivity	Escherichia coli
Host	Mouse
Clonality	Monoclonal
Immunogen	fd phages from E. coli F+ strain (JM109)
Specificity	Antibody Clone B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of G8P AEGDDPAKAAFDSLQASAT (See Kneissel et al.).
Formulation	10mM PBS, pH 7.4 State: Purified State: Lyophilized purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore in 1 ml distilled water
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. For prolonged storage of reconstituted antibody solution we strongly recommend addition of a stabilizing protein (e.g. 0.5% BSA) and a preservative (e.g. Thimerosal or Sodium Azide). Avoid repeated freezing and thawing.
Database Link	UniProt ID P69541
Background	M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
Synonyms	Gene 8 protein, Coat protein B, Major coat protein
Reference Data

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