pan Actin Mouse Monoclonal Antibody [Clone ID: 2G2]

Specifications

Product Data
Clone Name	2G2
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Cytological material. Immunofluorescence Microscopy (Only after methanol fixation). Immunohistochemistry on Frozen and Paraffin Sections: Ready to use. Incubation Time: 1h at RT (extended with paraffin). With Paraffin Embedded Sections, protease pretreatment is required prior to antibody application. When using antibody 2G2 in Immunolocalization studies the presence of nuclear Actin could be demonstrated e.g. in cultured cells after fixation with 4% paraformaldehyde. Immunostaining with cytoplasmic Actin was only positive after fixation with methanol (cf. Gansior et al., 1999).
Reactivity	Bovine, Chicken, Human, Rabbit, Xenopus
Host	Mouse
Clonality	Monoclonal
Immunogen	Profilin Actin complex from calf thymus Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA
Specificity	Specific for skeletal and non-muscle Actin isoforms (Mr 43,000). Teted Reactivities on Cultured Cell Lines: PtK2, SR-NRK, NRK-49F, L6 cells, Rat fibroblasts, NIH-3T3, mouse myoblast and myotube cells.
Formulation	State: Supernatant State: Liquid Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Background	The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
Reference Data

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