OATP2 (SLCO1B1) (+ OATP8) Mouse Monoclonal Antibody [Clone ID: mMDQ]

Specifications

Product Data
Clone Name	mMDQ
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Immunofluorescence Microscopy. Western blotting: For optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is not recommended. Alternatively, samples can be kept at 37°C for 30 min (See also Ref.1). Immunohistochemistry on Paraffin Sections (Only after microwave treatment): Use undiluted. For details See Ref. 1. Incubation Time: 1 h at RT, for Paraffin Sections overnight at 2-8°C.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic N-terminus (24 aa) of Human organic anion transporter OATP2 coupled to KLH
Specificity	In Western blot analyses of basolateral membrane fractions of human liver the monoclonal antibody mMDQ reacts with 90 kD and 120 kD proteins (after SDS-PAGE) representing OATP2 (also described as OATP-C) and OATP8, respectively. According to a recent new nomenclature these hepatocyte-specific proteins are identical to OATP1B1 and OATP1B3, respectively (See Ref.2). Using immunofluorescence microscopy mMDQ stains the sinusoidal (basolateral) membranes in frozen sections of human liver, hepatocellular carcinoma (See Ref.1). Reacts with OATP2 and OATP8, human organic anion transporters (90 kD, 120 kD, after SDS-PAGE). Reacts with Transfected HEK293 cells; negative with HepG2 and PLC.
Formulation	State: Supernatant State: Liquid Hybridoma Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	solute carrier organic anion transporter family member 1B1
Database Link	Entrez Gene 10599 Human UniProt ID Q9Y6L6
Synonyms	LST1, LST-1, OATP-2, OATPC, OATP-C, SLC21A6
Note	Protocol: Western Blotting (Ref.1) 1. For optimal detection of the glycosylated antigen sample boiling before SDS-PAGE is not recommended; alternatively, samples can be kept at 37°C for 30 min 2.50 µg of protein lysate should be loaded on each lane. 3. The usage of a PVDF-Membrane is recommended. 4. The usage of 10% milk powder in TTBS (Tris-buffered saline, pH 7.6, with 0.05% Tween 20) as blocking reagent. The antibody can be diluted 3-5 x
Reference Data
Protein Families	Druggable Genome, Transmembrane

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