Cytokeratin (basal cell) Mouse Monoclonal Antibody [Clone ID: RCK103]

Specifications

Product Data
Clone Name	RCK103
Applications	FC, IF, IHC, WB
Recommend Dilution	Western blot: 1/100-1/1000.  Immunohistochemistry on Frozen tissues: 1/100-1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.  Immunocytochemistry.  Immunofluorescence.  Flow Cytometry (1/100-1/200).
Reactivity	Canine, Chicken, Guinea Pig, Hamster, Human, Porcine, Quail, Rabbit, Rat, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	A mix of cell preparations containing Human cytokeratins.
Specificity	Clone RCK103 is a Cytokeratin antibody recognizing (amongst others) Cytokeratin 5. This monoclonal antibody stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Background	Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Reference Data

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