Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: RV202]

Specifications

Product Data
Clone Name	RV202
Applications	FC, IF, IHC, WB
Recommend Dilution	Immunoblotting. Flow Cytometry. Immunocytochemistry. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Recommended Dilutions: 1/100-1/200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 for Immunoblotting applications.
Reactivity	Canine, Chicken, Goat, Hamster, Human, Monkey, Mouse, Porcine, Rat, Xenopus, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeletal Vimentin extract of calf lens.
Specificity	RV202 reacts exclusively with Vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Synonyms	VIM
Reference Data

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