Neurofilament (NEFM) Mouse Monoclonal Antibody [Clone ID: RNF403]

Specifications

Product Data
Clone Name	RNF403
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunocytochemistry/Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissues. Recommended Dilutions: 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and for 1/100-1/500 for Immunoblotting.
Reactivity	Hamster, Human, Monkey, Rat, Xenopus
Host	Mouse
Clonality	Monoclonal
Immunogen	Neurofilament preparation of calf brain tissue.
Specificity	RNF403 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in small aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	neurofilament, medium polypeptide
Database Link	Entrez Gene 4741 Human UniProt ID P07197
Background	Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
Synonyms	Neurofilament medium polypeptide, NF-M, NEF3, NEFM, Neurofilament 3, (Neuronal Marker)
Reference Data

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