Neurofilament (NEFM) (phosphospecific) Mouse Monoclonal Antibody [Clone ID: RNF406]
CAT#: BM6012P
Neurofilament (NEFM) (phosphospecific) mouse monoclonal antibody, clone RNF406, Purified
Need it in bulk or conjugated?
Get a free quote
CNY 5,186.00
货期*
5周
规格
Cited in 1 publication. |
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Clone Name | RNF406 |
Applications | IHC, WB |
Recommend Dilution | Immunoblotting. Flow Cytometry. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissues. Recommended Dilutions: 1/50-1/100 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/500 for Immunoblotting. |
Reactivity | Guinea Pig, Hamster, Human, Monkey, Rabbit, Xenopus |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Neurofilament preparation of calf brain tissue. |
Specificity | RNF406 reacts exclusively with the phosphorylated isoform of the 160 kD neurofilament protein. Reacts with Human, Rabbit, Hamster, Monkey, Guinea Pig and Xenopus. |
Formulation | PBS with 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction. |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. |
Gene Name | neurofilament, medium polypeptide |
Database Link | |
Background | Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation. |
Synonyms | Neurofilament medium polypeptide, NF-M, NEF3, NEFM, Neurofilament 3, (Neuronal Marker) |
Reference Data |
Citations (1)
The use of this Antibodies has been cited in the following citations: |
---|
Mesenchymal stem cells from a hypoxic culture improve nerve regeneration
,Wang, JP;Liao, YT;Wu, SH;Chiang, ER;Hsu, SH;Tseng, TC;Hung, SC;,
J Tissue Eng Regen Med
,PubMed ID 32976700
[NEFM]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |
Customer
Reviews
Loading...