CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: RNL-1]

Specifications

Product Data
Clone Name	RNL-1
Applications	IF, IHC, WB
Recommend Dilution	Immunocytochemistry. Immunohistochemistry on frozen sections. Recommended dilutions: 1/100- 1/200 for immunohistochemistry with fluorochrome conjugated secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. For Immunoblotting applications the antibody has been used with previous lots at a 1/100-1/1000 dilution.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Small cell lung cancer cell line NCI-H82
Specificity	This clone was defined as a cluster I antibody during the Second International Workshop on Small Cell Lung Cancer (SCLC) Antibodies. RNL-1 recognizes the extracellular region of NCAM / CD56, which is present in small cell lung cancer (SCLC) and lung carcinoids. This antibody reacts with normal neural tissues and endocrine glands, such as pancreatic islet, pituitary gland and adrenal medulla. Expression was also found in Leydig cells of the testis, in the thyroid and in smooth-muscle cells of the small intestine, colon and bladder. The antibody is a valuable marker for the characterization of several neuroendocrine tumors.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	neural cell adhesion molecule 1
Database Link	Entrez Gene 4684 Human UniProt ID P13591
Background	NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM can be found in central and peripheral nerve cells, neuroendocrine tissues and at the surface of NK-cells. Also, NCAM is present in malignancies derived from these tissues and cells.
Synonyms	NCAM-1, N-CAM-1, NCAM
Reference Data

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