E Cadherin (CDH1) Mouse Monoclonal Antibody [Clone ID: MB2]

Specifications

Product Data
Clone Name	MB2
Applications	FC, IF, IHC, WB
Recommend Dilution	MB2 is useful for Flow cytometry, Immunoblotting, Immunocytochemistry on fixed cells (methanol fixation) and Immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Recommended Dilutions: Immunoblotting: 1/100-1/1000. Flow Cytometry: 1/100-1/200. Immunohistochemistry: 1/100-1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	MB2 is a mouse monoclonal IgG2b antibody derived by fusion of NS0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with MCF- 7/AZ cells expressing E-cadherin at their cell surface.
Specificity	MB2 recognizes both the 120 kD E-Cadherin and its 80 kD trypsin-resistant extracellular part. MB2 is a functional antibody in that it inhibits cell-cell adhesion.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	cadherin 1
Database Link	Entrez Gene 999 Human UniProt ID P12830
Background	Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin αEβ7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently down-regulated, which is consistent with its function of an invasion suppressor in normal epithelia.
Synonyms	Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO
Reference Data

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