Sele (CR2 Region) Rat Monoclonal Antibody [Clone ID: UZ6]

Specifications

Product Data
Clone Name	UZ6
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	ELISA. Immunoblotting. Immunoprecipitation. Flow Cytometry: 1/500. Immunofluorescence. Immunocytochemistry. Immunohistochemistry on Fozen Sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
Specificity	UZ6 recognizes a conformational epitope in the CR2 region in murine E-selectin. UZ6 partially inhibits leukocyte adhesion to murine activated endothelial cells.
Formulation	PBS with 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	selectin, endothelial cell
Database Link	Entrez Gene 20339 Mouse UniProt ID Q00690
Background	Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
Synonyms	SELE, ELAM1, LECAM2
Reference Data

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