Tubulin (TUBA1B) (Loading Control) Mouse Monoclonal Antibody [Clone ID: TU-01]

Specifications

Product Data
Clone Name	TU-01
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	Western Blotting (Reducing conditions): Recommended Dilutions: 1-2 µg/ml. Incubation Time: 60 min at RT. Positive Control: HPB-ALL peripheral blood leukemia cell lysate (incubation 60 min). Porcine brain lysate (incubation 90 min). Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Flow cytometry: Recommended dilution: 1-4 μg/ml, intracellular staining. Immunocytochemistry: Recommended Dilutions: DY547 conjugate: 2-3 µg/ml, FITC conjugate: 3 µg/ml. Staining Technique: fixed and permeabilized cells. Immunohistochemistry on Paraffin Sections: Recommended Dilutions: 5 µg/ml. Positive Control: Heart. Clone TU-01 has also been described to work in ELISA and Immunoprecipitation.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Immunogen	Fraction of Tubulin purified from Pig brain by two cycles of polymerization-depolymerization
Specificity	The antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha Tubulin. Reacts with all species (recognized epitope conserved within all species).
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified IgG fraction (>95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Precipitation Methods
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	tubulin alpha 1b
Database Link	Entrez Gene 10376 Human UniProt ID P68363
Background	The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening; this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or morecommonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight about 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structuraldomain.
Synonyms	Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Gap junction, Pathogenic Escherichia coli infection

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