IL4R Mouse Monoclonal Antibody [Clone ID: 25463 (49/20)]

Specifications

Product Data
Clone Name	25463 (49/20)
Applications	ELISA, FC, FN, IHC, WB
Recommend Dilution	Flow Cytometry: 1/10-1/40. ELISA: 1/250-1/1000. Western Blot: 1/125-1/500. This antibody will detect Human IL-4 R under non-reducing conditions. Functional Assays. Immunohistochemistry on Paraffin Sections: 1/20-1/60.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Sf 21-derived, recombinant Human interleukin 4 Receptor, extracellular domain.
Specificity	This antibody is specific for Interleukin 4 Receptor, the transmembrane ligand-binding subunit of the heterodimeric IL-4 receptor complex. This antibody blocks cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/ml in the presence of 0.2 ng/ml of IL-4 using the factor dependant Human erythroleukemic cell line, TF-1.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Ascites with 0.5% Trehalose
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	interleukin 4 receptor
Database Link	Entrez Gene 3566 Human UniProt ID P24394
Background	IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.
Synonyms	Interleukin-4 receptor alpha chain, IL-4R-alpha, IL4RA, 582J2.1, IL4 Receptor alpha
Reference Data

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