Salivary alpha amylase (AMY1A) Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1/20-1/200. ELISA: 1/500-1/3000. In a simple Indirect ELISA with 400 ng of Human salivary amylase bound to the plate, the 50% OD was observed at an antibody dilution of 1/5K. Under the same conditions, the end point was observed at 1/500K.
Reactivity	Fungi, Human, Porcine
Host	Sheep
Clonality	Polyclonal
Immunogen	Highly pure Human Salivary Amylase.
Specificity	Human salivary Amylase: 100% Porcine pancreatic Amylase 50% Amylase from Aspergillus oryzae 10%
Formulation	PBS pH 7.2 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in alqiuots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	amylase, alpha 1A (salivary)
Database Link	Entrez Gene 276 Human UniProt ID P04745
Background	Amylases are secreted proteins that hydrolyze 1,4 alpha glucoside bonds in oligosaccharides and polysaccharides. They catalyze the first step in digestion of dietary starch and glycogen. Alternative splicing results in multiple transcript variants encoding the same protein. Salivary Amylase is a digestive enzyme secreted by salivary glands, it consists of a single polypeptide chain with a molecular weight of 56kDa (family B) or 62kDa (family A), the difference in molecular weight being due to the presence or absence of a carbohydrate moiety. The total serum concentration of amylase is about 120g/L of which about 50% is salivary amylase. Although salivary amylase and pancreatic amylase share 95% amino acid sequence homology, they differ in molecular size, isoelectricpoint and antigenic properties.
Synonyms	Salivary alpha-amylase, AMY1A
Reference Data

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