FITC Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	ELISA.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Fluorescein isothiocyanate conjugated to keyhole limpet haemocyanin.
Specificity	This antibody is specific for Fluorescein Isothiocyanate (FITC) and cross reacts with Fluoresceinamine-BSA and Fluorescein-BSA.
Formulation	Phosphate buffered saline State: Ig Fraction State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Should this product contain a precipitate we recommend microcentrifugation before use. Avoid repeated freezing and thawing.
Background	FITC (Fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
Synonyms	Fluorescein Isothiocyanate
Reference Data

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