Desmoglein 2 (DSG2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot: 1/3000 (ECL method). Immunohistochemistry on Cryostat Sections: 1/100 for 1h at RT.  For better resolution on frozen sections preincubation (directly after fixation with –20°C methanol) with 0.05 - 0.5% Triton X- 100, for 5-10 min, depending on tissue type, is recommended (See Ref.3).  Recommended Positive Control: CaCo-2, HaCaT.  Reactivities on Cultured Cell Lines: Many, including CaCo-2, HaCaT, MCF-7.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant peptide (extracellular repeat domain E2 of Human Desmoglein 2)
Specificity	Antibody recognizes desmoglein 2 present in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low.
Formulation	State: Serum State: Liquid serum Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	desmoglein 2
Database Link	Entrez Gene 1829 Human UniProt ID Q14126
Background	Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.
Synonyms	DSG2, CDHF5,HDGC
Reference Data

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