Cytokeratin 8 (KRT8) (+ KRT18) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Immunoblotting: 1/2000 for Western blotting. Cytological material. Immunohistochemistry on Frozen Tissue: 1/100. Immunohistochemistry on Paraffin-Embedded Tissue: 1/50 (microwave treatment recommended). Incubation Time: 1 h at RT, extended with Paraffin.
Reactivity	Bovine, Chicken, Hamster, Human, Mouse, Rat, Xenopus
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Keratin K8/K18 filaments, reconstituted from purified Bovine keratins K8 and K18
Specificity	Reactive Polypeptide: Mr 52 500 and 45 500 Keratins K8 and K18 of simple type epithelium. Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma.
Formulation	State: Serum State: Liquid stabilized antiserum Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 8
Database Link	Entrez Gene 16691 MouseEntrez Gene 25626 RatEntrez Gene 3856 Human UniProt ID P05787
Background	Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Synonyms	KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A
Reference Data
Protein Families	Druggable Genome

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