Cingulin (CGN) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunofluorescence Microscopy: 1/100. Dilute immedialtely with PBS before use. Immunohistochemistry on Frozen Sections On cultured cells initial fixation with formaldehyde is recommended for optimal staining. Immunohistochemistry on Paraffin Sections (Only after microwave treatment). Positive Control: Total cell lysate of e.g. CaCo-2 cells.
Reactivity	Bovine, Human, Mouse, Rabbit, Xenopus
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Full length GST-fusion protein of Human Cingulin
Specificity	The antibody reacts specifically with cingulin (a ca.150 kD polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas) of transitional epithelia (e.g. bladder epithelium) and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina). It is negative with endothelia of vessels and capillaries (e.g. in heart and brain). Tested Reactivities on Cultured Cell Lines: Carcinoma cell lines of Human (e.g. MCF-7, Caco-2, PLC, A-431), Bovine (MDBK, BMGE) and Canine (MDCK) origin.
Formulation	State: Serum State: Liquid Stabilized Antiserum with 0.5% BSA and 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	cingulin
Database Link	Entrez Gene 57530 Human UniProt ID Q9P2M7
Background	Cingulin, a polypeptide of 126.5 kD (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5 (very similar to that of symplekin, another junctional protein)
Synonyms	CGN, KIAA1319
Reference Data
Protein Pathways	Tight junction

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