Insulin (INS) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	ELISA. Immunohistochemistry on Frozen and Paraffin Sections: Proteolytic treatment is not required, optimal results have been obtained by using the autoclave or microwave retrieval method (with citrate buffer). Working Dilutions: For immunohistochemistry dilute 1/50-1/100 (with PBS or TBS immediately before use), based on the indirect method, with a normal ABC method the dilution can be up to 5x higher when using the microwave retrieval method. Recommended Positive Control: Pancreas.
Reactivity	Ferret, Human, Porcine, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Porcine Insulin
Specificity	The antibody detects Insulin which is produced by the B-cells of the pancreatic islets. Antibody BP5053 can be used for the diagnosis of pancreatic B-cell tumors. Antigen localization: cytoplasm, extracellular.
Formulation	State: Serum State: Liquid Serum
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	insulin
Database Link	Entrez Gene 3630 Human UniProt ID P01308
Background	Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
Synonyms	INS
Reference Data

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