Cytokeratin 1 (KRT1) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Cytological Material. Western blotting: 1/3,000 (ECL). Immunohistochemistry on Frozen Sections: 1/200. Immunohistochemistry on Paraffin Embedded Material: 1/100 (after microwave treatment or trypsin). Incubation Time: 1 h at RT, extended with paraffin sections. Positive Control: Human foot sole.
Reactivity	Human, Mouse
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptide of Human keratin K1 (formerly also designated Cytokeratin 1) coupled to KLH
Specificity	Reacts with Basic keratin K1 (Mr 66 000). Specific for keratin K1 in suprabasal layers of cornified squamous epithelia, e.g. the suprabasal layer of scalp and foot sole. Negative with non-keratinizing stratified and non-stratified epithelia (e.g. esophagus, duodenum)!
Formulation	State: Serum State: Liquid antiserum containing 0.09% Sodium Azide and 0.5% BSA as stabilizer.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	keratin 1
Database Link	Entrez Gene 3848 Human UniProt ID P04264
Background	Cytokeratin 1 is a type II Cytokeratin. The type II Cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II Cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.
Synonyms	Keratin-1, KRT1, K1, Cytokeratin 1, Hair alpha protein, 67 kDA cytokeratin, KRTA
Reference Data

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