COL1A1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R
Recommend Dilution	RIA. ELISA: 1/400 (OD ≥ 500). Immunoblotting (Western blot). Immunofluorescence (1/80). Immunohistochemistry on Paraffin embedded tissues: 1/100-1/500.  Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: 60 min at RT or 2-8°C overnight. Positive Control: Bovine skin.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified Collagen type I from Bovine skin.
Specificity	Bovine Collagen type I: 100%. Bovine Collagen II, IX, XI: < 0.1% Sheep Collagen type I: 50% (IHC estimated) Bovine Fibronectin: < 0.1% Human Collagen type I: < 2% Chicken Collagen type I: < 0.1% (Estimates except Sheep Collagen I from solid phase RIA at dilution 1/500). Even though cross-reactivity with Human is low in RIA, the antibody has to be expected to be cross-reactivive to Human Collagen type I as well as to Pig Collagen type I in IHC.
Formulation	PBS without BSA and preservatives State: Purified State: Lyophilized purified Ig fraction Stabilizer: None Preservative: None
Reconstitution Method	Restore with 0.1 ml (BP8000S) or 0.5 ml (BP8000) distilled water. For further dilution use appropriate antibody diluent.
Concentration	~1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 282187 Bovine UniProt ID P02453
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I Collagen (95 kDa) usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues.
Synonyms	COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data

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