beta III Tubulin (TUBB3) Mouse Monoclonal Antibody [Clone ID: OTI5H2]

Specifications

Product Data
Clone Name	OTI5H2
Applications	WB
Recommend Dilution	WB 1:1000~2000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-150 of human TUBB3 (NP_006077) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	50.3 kDa
Gene Name	tubulin beta 3 class III
Database Link	NP_006077 Entrez Gene 22152 MouseEntrez Gene 246118 RatEntrez Gene 10381 Human UniProt ID Q13509
Background	Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules.
Synonyms	beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways	Gap junction, Pathogenic Escherichia coli infection

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