ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI1B10]

Specifications

Product Data
Clone Name	OTI1B10
Applications	IF, WB
Recommend Dilution	WB: 1:500, IF (1:100)
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant protein expressed in E.coli corresponding to amino acids 1-331 of human DFF45
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	36.3 kDa
Gene Name	DNA fragmentation factor subunit alpha
Database Link	NP_004392 Entrez Gene 1676 Human UniProt ID O00273
Background	Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene
Synonyms	DFF-45; DFF1; ICAD
Reference Data
Protein Pathways	Apoptosis

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