GATA4 Mouse Monoclonal Antibody [Clone ID: OTI9F9]

Specifications

Product Data
Clone Name	OTI9F9
Applications	IF, IHC, WB
Recommend Dilution	WB 1:200~500, IHC 1:50, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 296-442 of human GATA4 (NP_002043) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	44.4 kDa
Gene Name	GATA binding protein 4
Database Link	NP_002043 Entrez Gene 14463 MouseEntrez Gene 54254 RatEntrez Gene 2626 Human UniProt ID P43694
Background	GATA4 encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.
Synonyms	ASD2; TACHD; TOF; VSD1
Reference Data
Protein Families	Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors

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