HES1 Mouse Monoclonal Antibody [Clone ID: OTI4H1]

Specifications

Product Data
Clone Name	OTI4H1
Applications	IF, IHC, WB
Recommend Dilution	WB 1:1000; IHC 1:50; RPPA 1:75; IF (1:100)
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	29.4 kDa
Gene Name	hes family bHLH transcription factor 1
Database Link	NP_005515 Entrez Gene 15205 MouseEntrez Gene 29577 RatEntrez Gene 3280 Human UniProt ID Q14469
Background	This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Synonyms	bHLHb39; HES-1; HHL; HRY
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
Protein Pathways	Maturity onset diabetes of the young, Notch signaling pathway

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