Carbonic Anhydrase IX (CA9) Mouse Monoclonal Antibody [Clone ID: OTI1G7]

Specifications

Product Data
Clone Name	OTI1G7
Applications	FC, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:50, Flow 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length protein expressed in 293T cell transfected with human CA9 expression vector
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	49.7 kDa
Gene Name	carbonic anhydrase 9
Database Link	NP_001207 Entrez Gene 768 Human UniProt ID Q16790
Background	Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-basebalance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
Synonyms	CAIX; MN
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Nitrogen metabolism

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