Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI10D7]

Specifications

Product Data
Clone Name	OTI10D7
Applications	FC, IF, WB
Recommend Dilution	IF 1:100, FLOW 1:100, WB: 1:200
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	73.5 kDa
Gene Name	heat shock protein family A (Hsp70) member 9
Database Link	NP_004125 Entrez Gene 15526 MouseEntrez Gene 291671 RatEntrez Gene 3313 Human UniProt ID P38646
Background	This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
Synonyms	CSA; GRP-75; GRP75; HEL-S-124m; HSPA9B; MOT; MOT2; MTHSP75; PBP74
Reference Data
Protein Families	Stem cell - Pluripotency
Protein Pathways	RNA degradation

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