GBE1 Mouse Monoclonal Antibody [Clone ID: OTI5B7]

Specifications

Product Data
Clone Name	OTI5B7
Applications	FC, IHC, IP, WB
Recommend Dilution	WB 1:2000, IHC 1:50, FLOW 1:100, IP 2ug/500ul
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	80.3 kDa
Gene Name	1,4-alpha-glucan branching enzyme 1
Database Link	NP_000149 Entrez Gene 2632 Human UniProt ID Q04446
Background	The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq]
Synonyms	APBD; GBE; GSD4
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Starch and sucrose metabolism

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